You are currently browsing the category archive for the ‘papers’ category.

This post is the fifth in a series of five posts related to the paper “Melsted, Booeshaghi et al., Modular and efficient pre-processing of single-cell RNA-seq, bioRxiv, 2019“. The posts are:

The following passage about Beethoven’s fifth symphony was written by one of my favorite musicologists:

“No great music has ever been built from an initial figure of four notes. As I have said elsewhere, you might as well say that every piece of music is built from an initial figure of one note. You may profitably say that the highest living creatures have begun from a single nucleated cell. But no ultra-microscope has yet unraveled the complexities of the single living cell; nor, if the spectroscope is to be believed, are we yet very full informed of the complexities of a single atom of iron : and it is quite absurd to suppose that the evolution of a piece of music can proceed from a ‘simple figure of four notes’ on lines in the least resembling those of nature.” – Donald Francis Tovey writing about Beethoven’s Fifth Symphony in Essays in Musical Analysis Volume I, 1935.

This passage conveys something true about Beethoven’s fifth symphony: an understanding of it cannot arise from a limited fixation on the famous four note motif. As far as single-cell biology goes, I don’t know whether Tovey was familiar with Theodor Boveri‘s sea urchin experiments, but he certainly hit upon a scientific truth as well: single cells cannot be understood in isolation. Key to understanding them is context (Eberwine et al., 2013).

RNA velocity, with roots in the work of Zeisel et al., 2011, has been recently adapted for single-cell RNA-seq by La Manno et al. 2018, and provides much needed context for interpreting the transcriptomes of single-cells in the form of a dynamics overlay. Since writing a review about the idea last year (Svensson and Pachter, 2019), I’ve become increasingly convinced that the method, despite relying on sparse data, numerous very strong model assumptions, and lots of averaging, is providing meaningful biological insight. For example, in a recent study of spermatogonial stem cells (Guo et al. 2018), the authors describe two “unexpected” transitions between distinct states of cells that are revealed by RNA velocity analysis (panel a from their Figure 6, see below):

Producing an RNA velocity analysis currently requires running the programs Cell Ranger followed by velocyto. These programs are both very slow. Cell Ranger’s running time scales at about 3 hours per hundred million reads (see Supplementary Table 1 Melsted, Booeshaghi et al., 2019). The subsequent velocyto run is also slow. The authors describe it as taking “approximately 3 hours” but anecdotally the running time can be much longer on large datasets. The programs also require lots of memory.

To facilitate rapid and large-scale RNA velocity analysis, in Melsted, Booeshaghi et al., 2019  we describe a kallisto|bustools workflow that makes possible efficient RNA velocity computations at least an order of magnitude faster than with Cell Ranger and velocyto. The work, a tour-de-force of development, testing and validation, was primarily that of Sina Booeshaghi. Páll Melsted implemented the bustools capture command and Kristján Hjörleifsson assisted with identifying and optimizing the indices for pseudoalignment. We present analysis on two datasets in the paper. The first is single-cell RNA-seq from retinal development recently published in Clark et al. 2019. This is a beautiful paper- and I don’t mean just in terms of the results. Their data and results are extremely well organized making their paper reproducible. This is so important it merits a shout out 👏🏾

See Clark et al. 2019‘s  GEO GSE 118614 for a well-organized and useful data share.

The figure below shows RNA velocity vectors overlaid on UMAP coordinates for Clark et al.’s 10 stage time series of retinal development (see cell [8] in our python notebook):

An overlap on the same UMAP with cells colored by type is shown below:

Clark et al. performed a detailed pseudotime analysis in their paper, which successfully identified genes associated with cell changes during development. This is a reproduction of their figure 2:

We examined the six genes from their panel C from a velocity point of view using the scvelo package and the results are beautiful:

What can be seen with RNA velocity is not only the changes in expression that are extracted from pseudotime analysis (Clark et al. 2019 Figure 2 panel C), but also changes in their velocity, i.e. their acceleration (middle column above). RNA velocity adds an interesting dimension to the analysis.

To validate that our RNA velocity workflow provides results consistent with velocyto, we performed a direct comparison with the developing human forebrain dataset published by La Manno et al. in the original RNA velocity paper (La Manno et al. 2018 Figure 4).

The results are concordant, not only in terms of the displayed vectors, but also, crucially, in the estimation of the underlying phase diagrams (the figure below shows a comparison for the same dataset; kallisto on the left, Cell Ranger + velocyto on the right):

Digging deeper into the data, one difference we found between the workflows (other than speed) is the number of reads counts. We implemented a simple strategy to estimate the required spliced and unspliced matrices that attempts to follow the one described in the La Manno et al. paper, where the authors describe the rules for characterizing reads as spliced vs. unspliced as follows:

1. A molecule was annotated as spliced if all of the reads in the set supporting a given molecule map only to the exonic regions of the compatible transcripts.
2. A molecule was annotated as unspliced if all of the compatible transcript models had at least one read among the supporting set of reads for this molecule mapping that i) spanned exon-intron boundary, or ii) mapped to the intron of that transcript.

In the kallisto|bustools workflow this logic was implemented via the bustools capture command which was first use to identify all reads that were compatible only with exons (i.e. there was no pseudoalignment to any intron) and then all reads that were compatible only with introns  (i.e. there was no pseudoalignment completely within an exon). While our “spliced matrices” had similar numbers of counts, our “unspliced matrices” had considerably more (see Melsted, Booeshaghi et al. 2019 Supplementary Figure 10A and B):

To understand the discrepancy better we investigated the La Manno et al. code, and we believe that differences arise from the velocyto package logic.py code in which the same count function

###### def count(self, molitem: vcy.Molitem, cell_bcidx: int, dict_layers_columns: Dict[str, np.ndarray], geneid2ix: Dict[str, int])

appears 8 times and each version appears to implement a slightly different “logic” than described in the methods section.

A tutorial showing how to efficiently perform RNA velocity is available on the kallisto|bustools website. There is no excuse not to examine cells in context.

This post is the fourth in a series of five posts related to the paper “Melsted, Booeshaghi et al., Modular and efficient pre-processing of single-cell RNA-seq, bioRxiv, 2019“. The posts are:

1. Near-optimal pre-processing of single-cell RNA-seq
2. Single-cell RNA-seq for dummies
3. How to solve an NP-complete problem in linear time
4. Rotating the knee (plot) and related yoga
5. High velocity RNA velocity

The “knee plot” is a standard single-cell RNA-seq quality control that is also used to determine a threshold for considering cells valid for analysis in an experiment. To make the plot, cells are ordered on the x-axis according to the number of distinct UMIs observed. The y-axis displays the number of distinct UMIs for each barcode (here barcodes are proxies for cells). The following example is from Aaron Lun’s DropletUtils vignette:

A single-cell RNA-seq knee plot.

High quality barcodes are located on the left hand side of the plot, and thresholding is performed by identifying the “knee” on the curve. On the right hand side, past the inflection point, are barcodes which have relatively low numbers of reads, and are therefore considered to have had failure in capture and to be too noisy for further analysis.

In Melsted, Booeshaghi et al., Modular and efficient pre-processing of single-cell RNA-seq, bioRxiv, 2019, we display a series of plots for a benchmark panel of 20 datasets, and the first plot in each panel (subplot A)is a knee plot. The following example is from an Arabidopsis thaliana dataset (Ryu et al., 2019; SRR8257100)

Careful examination of our plots shows that unlike the typical knee plot made for single-cell RNA-seq , ours has the x- and y- axes transposed. In our plot the x-axis displays the number of distinct UMI counts, and the y-axis corresponds to the barcodes, ordered from those with the most UMIs (bottom) to the least (top). The figure below shows both versions of a knee plot for the same data (the “standard” one in blue, our transposed plot in red):

Why bother transposing a plot?

We begin by observing that if one ranks barcodes according to the number of distinct UMIs associated with them (from highest to lowest), then the rank of a barcode with x distinct UMIs is given by f(x) where

$f(x) = |\{c:\# \mbox{UMIs} \in c \geq x\}|$.

In other words, the rank of a barcode is interpretable as the size of a certain set. Now suppose that instead of only measurements of RNA molecules in cells, there is another measurement. This could be measurement of surface protein abundances (e.g. CITE-seq or REAP-seq), or measurements of sample tags from a multiplexing technology (e.g. ClickTags). The natural interpretation of #distinct UMIs as the independent variable and  the rank of a barcode as the dependent variable is now clearly preferable. We can now define a bivariate function f(x,y) which informs on the number of barcodes with at least x RNA observations and tag observations:

$f(x,y) = |\{c:\# \mbox{UMIs} \in c \geq x \mbox{ and} \# \mbox{tags} \in c \geq y \}|$.

Nadia Volovich, with whom I’ve worked on this, has examined this function for the 8 sample species mixing experiment from Gehring et al. 2018. The function is shown below:

Here the x-axis corresponds to the #UMIs in a barcode, and the y-axis to the number of tags. The z-axis, or height of the surface, is the f(x,y) as defined above.  Instead of thresholding on either #UMIs or #tags, this “3D knee plot” makes possible thresholding using both (note that the red curve shown above corresponds to one projection of this surface).

Separately from the issue described above, there is another subtle issue with the knee plot. The x-axis (dependent) variable really ought to display the number of molecules assayed rather than the number of distinct UMIs. In the notation of Melsted, Booeshaghi et al., 2019 (see also the blog post on single-cell RNA-seq for dummies), what is currently being plotted is |supp(I)|, instead of |I|. While |I| cannot be directly measured, it can be inferred (see the Supplementary Note of Melsted, Booeshaghi et al., 2019), where the cardinality of I is denoted by k (see also Grün et al,, 2014). If d denotes the number of distinct UMIs for a barcode and n the effective number of UMIs , then k can be estimated by

$\hat{k} = \frac{log(1-\frac{d}{n})}{log(1-\frac{1}{n})}$.

The function estimating k is monotonic so for the purpose of thresholding with the knee plot it doesn’t matter much whether the correction is applied, but it is worth noting that the correction can be applied without much difficulty.

This post is the third in a series of five posts related to the paper “Melsted, Booeshaghi et al., Modular and efficient pre-processing of single-cell RNA-seq, bioRxiv, 2019“. The posts are:

1. Near-optimal pre-processing of single-cell RNA-seq
2. Single-cell RNA-seq for dummies
3. How to solve an NP-complete problem in linear time
4. Rotating the knee (plot) and related yoga
5. High velocity RNA velocity

There is a million dollar prize on offer for a solution to the P vs. NP problem, so it’s understandable that one may wonder whether this blog post is an official entry. It is not.

The title for this post was inspired by a talk presented by David Tse at the CGSI 2017 meeting where he explained “How to solve NP-hard assembly problems in linear time“. The gist of the talk was summarized by Tse as follows:

“In computational genomics there’s been a lot of problems where the formulation is combinatorial optimization. Usually they come from some maximum likelihood formulation of some inference problem and those problems end up being mostly NP-hard. And the solution is typically to develop some heuristic way of solving the NP-hard problem. What I’m saying here is that actually there is a different way of approaching such problems. You can look at them from an information point of view.”

Of course thinking about NP-hard problems from an information point of view does not provide polynomial algorithms for them. But what Tse means is that information-theoretic insights can lead to efficient algorithms that squeeze the most out of the available information.

One of the computational genomics areas where an NP-complete formulation for a key problem was recently proposed is in single-cell RNA-seq pre-processing. After RNA molecules are captured from cells, they are amplified by PCR, and it is possible, in principle, to account for the PCR duplicates of the molecules by making use of unique molecular identifiers (UMIs). Since UMIs are (in theory) unique to each captured molecule, but identical among the PCR duplicates of that captured molecule, they can be used to identify and discard the PCR duplicates. In practice distinct captured molecules may share the same UMI causing a collision, so it can be challenging to decide when to “collapse” reads to account for PCR duplicates.

In the recent paper Srivastava et al. 2019, the authors developed a combinatorial optimization formulation for collapsing. They introduce the notion of “monochromatic arborescences” on a graph, where these objects correspond to what is, in the language of the previous post, elements of the set C. They explain that the combinatorial optimization formulation of UMI collapsing in this framework is to find a minimum cardinality covering of a certain graph by monochromatic arboresences. The authors then prove the following theorem, by reduction from the dominating set decision problem:

Theorem [Srivastava, Malik, Smith, Sudbery, Patro]: Minimum cardinality covering by monochromatic arborescences is NP-complete.

Following the standard practice David Tse described in his talk, the authors then apply a heuristic to the challenging NP-complete problem. It’s all good except for one small thing. The formulation is based on an assumption, articulated in Srivastava et al. 2019 (boldface and strikethrough is mine):

…gene-level deduplication provides a conservative approach and assumes that it is highly unlikely for molecules that are distinct transcripts of the same gene to be tagged with a similar UMI (within an edit distance of 1 from another UMI from the same gene). However, entirely discarding transcript-level information will mask true UMI collisions to some degree, even when there is direct evidence that similar UMIs must have arisen from distinct transcripts. For example, if similar UMIs appear in transcript-disjoint equivalence classes (even if all of the transcripts labeling both classes belong to the same gene), then they cannot have arisen from the same pre-PCR molecule. Accounting for such cases is especially true [important] when using an error-aware deduplication approach and as sequencing depth increases.

The one small thing? Well… the authors never checked whether the claim at the end, namely that “accounting for such cases is especially important”, is actually true. In our paper “Modular and efficient pre-processing of single-cell RNA-seq” we checked. The result is in our Figure 1d:

Each column in the figure corresponds to a dataset, and the y-axis shows the distribution (over cells) of the proportion of counts one can expect to lose if applying naïve collapsing to a gene. Naïve collapsing here means that two reads with the same UMI are considered to have come from the same molecule. The numbers are so small we had to include an inset in the top right. Basically, it almost never happens that there is “direct evidence that similar UMIs must have arisen from distinct transcripts”. If one does observe such an occurrence, it is almost certainly an artifact of missing annotation. In fact, this leads to an…

💡 Idea: prioritize genes with colliding UMIs for annotation correction. The UMIs directly highlight transcripts that are incomplete. Maybe for a future paper, but returning to the matter at hand…

Crucially, the information analysis shows that there is no point in solving an NP-complete problem in this setting. The naïve algorithm not only suffices, it is sensible to apply it. And the great thing about naïve collapsing is that it’s straightforward to implement and run; the algorithm is linear. The Srivastava et al. question of what is the “minimum number of UMIs, along with their counts, required to explain the set of mapped reads” is a precise, but wrong question. In the words of John Tukey: “Far better an approximate answer to the right question, which is often vague, than an exact answer to the wrong question, which can always be made precise.”

The math behind Figure 1d is elementary but interesting (see the Supplementary Note of our paper). We work with a simple binomial model which we justify based on the data. For related work see Petukhov et al. 2018. One interesting result that came out of our calculations (work done with Sina Booeshaghi), is an estimate for the effective number of UMIs on each bead in a cell. This resulted in Supplementary Figure 1:

The result is encouraging. While the number of UMIs on a bead is not quite $4^L$ where L is the length of the UMI (theoretical maximum shown by dashed red line for v2 chemistry and solid red line for v3 chemistry), it is nevertheless high. We don’t know whether the variation is a result of batch effect, model mis-specification, or other artifacts; that is an interesting question to explore with more data and analysis.

As for UMI collapsing, the naïve algorithm has been used for almost every experiment to date as it is the method that was implemented in the Cell Ranger software, and subsequently adopted in other software packages. This was done without any consideration of whether it is appropriate. As the Srivastava et al. paper shows, intuition is not to be relied upon, but fortunately, in this case, the naïve approach is the right one.

This post is the second in a series of five posts related to the paper “Melsted, Booeshaghi et al., Modular and efficient pre-processing of single-cell RNA-seq, bioRxiv, 2019“. The posts are:

A few months ago, while working on the kallisto | bustools project, some of us in the lab were discussing various aspects of single-cell RNA-seq technology when the conversation veered into a debate over the meaning of some frequently used words and phrases in the art: “library complexity”, “library size”, “sensitivity”, “capture rate”, “saturation”, “number of UMIs”, “bork bork bork” etc. There was some sense of confusion. I felt like a dummy because even after working on RNA-seq for more than a decade, I was still lacking language and clarity about even the most basic concepts. This was perhaps not entirely my fault. Consider, for example, that the phrase “library size” is used to mean “the number of molecules in a cDNA library” by some authors, and the “number of reads sequenced” by others.

Since we were writing a paper on single-cell RNA-seq pre-processing that required some calculations related to the basic concepts (libraries, UMIs, and so on), we decided to write down notation for the key objects. After some back-and-forth, Sina Booeshaghi and I ended up drafting the diagram below that summarizes the sets of objects in a single-cell RNA-seq experiment, and the maps that relate them:

Structure of a single-cell RNA-seq experiment.

Each letter in this diagram is a set. The ensemble of RNA molecules contained within a single cell is denoted by R. To investigate R, a library (L) is constructed from the set of molecules captured from R (the set C). Typically, L is the result of of various fragmentation and amplification steps performed on C, meaning each element of C may be observed in L with some multiplicity. Thus, there is an inclusion map from C to L (arrow with curly tail), and an injection from C to R (arrows with head and tail). The library is interrogated via sequencing of some of the molecules in L, resulting in a set F of fragments. Subsequently, the set F is aligned or pseudoaligned to create a set B, which in our case is a BUS file. Not every fragment F is represented in B, hence the injection, rather than bijection, from B to F, and similarly from F to L. The set T consists of transcripts that correspond to molecules in C that were represented in B. Note that $|R| \geq |C| \geq |T|$. Separately, the set U consists of the unique molecular identifiers (UMIs) available to label molecules from the cell, and I is a multiset of UMIs associated with the molecules in T. Importantly, the data from an experiment consists of F, together with the support of I. The support of I means the number of distinct objects in I, and is denoted by |supp(I)|. The common term is “number of distinct UMIs”.

The diagram has three distinct parts. The sets on the top (L, F, B) are “lifted” from  and by PCR. Without PCR one would be in an the ideal situation of measuring C directly to produce T, which would then be used to directly draw inferences about R. This is the hope for direct RNA sequencing, a technology that is promising but that cannot yet be applied at the scale of cDNA based methods. The sets U and I are intended to be seen as orthogonal to the rest of the objects. They relate to the UMIs which, in droplet single-cell RNA-seq technology, are delivered via beads. While the figure was designed to describe single-cell RNA-seq, it is quite general and possibly a useful model for many sequence census assays.

So what is all this formality good for? Nothing in this setup is novel; any practitioner working with single-cell RNA-seq already knows what the ingredients for the technology are. However I do think there is some trouble with the language and meaning of words, and hopefully having names and labels for the relevant sets can help in communication.

The questions

With some notation at hand, it is possible to precisely articulate some of the key technical questions associated with a single-cell RNA-seq experiment:

• The alignment (or pseudoalignment) problem: compute B from F.
• The pre-processing problem: what is the set ?
• What is the library richness/complexity, i.e. what is |supp(L)|?
• What is the sensitivity, i.e. what is $\frac{|C|}{|R|}$?
• In droplet based experiments, what are the number of UMIs available to tag molecules in a cell, i.e. what is |U|?

These basic questions are sometimes confused with each other. For example, the capture rate refers to the proportion of cells from a sample that are captured in an experiment and should not be confused with sensitivity. The |supp(L)| is a concept that is natural to refer to when thinking about a cDNA library. Note that the “library size”, referred to in the beginning of this post, is used by molecular biologists to naturally mean |L|, and not |F| (this confusion was unfortunately disseminated by the highly influential RNA-seq papers Anders and Huber, 2010 and Robinson and Oshlack, 2010) . The support of another set, |supp(I)|, is one that is easy to measure but precisely because I is a multiset, $|I| \neq |supp(I)|$, and there is considerable confusion about this fact. The number of distinct UMIs, |supp(I)|, is frequently used in lieu of the set whose size is being referred to, namely |I| (this is the case when “knee plots” are made, a topic for the fourth blog post in this series). Similarly, |U| is usually not estimated, and the number $4^L$ where $L$ is the length of the UMIs is used in its stead. This is partly intellectual laziness but partly, I think, the lack of standard notation to refer to the objects in single-cell RNA-seq experiments.

This diagram in this post is just step 0 in discussing single-cell RNA-seq. There is a lot more subtlety and nuance in understanding and interpreting experiments (see Introduction to single-cell RNA-seq technologies). ∎

This post is the first in a series of five posts related to the paper “Melsted, Booeshaghi et al., Modular and efficient pre-processing of single-cell RNA-seq, bioRxiv, 2019“. The posts are:

1. Near-optimal pre-processing of single-cell RNA-seq
2. Single-cell RNA-seq for dummies
3. How to solve an NP-complete problem in linear time
4. Rotating the knee (plot) and related yoga
5. High velocity RNA velocity

During the past few years computational biologists have expended enormous effort in developing tools for processing and analyzing single-cell RNA-seq. This post describes yet another: the kallisto|bustools workflow for pre-processing single-cell RNA-seq. A preprint describing the method (was recently posted on the bioRχiv.

Number of single-cell RNA-seq tools (from the scRNA-tools catalog).

Given that there are so many programs, a natural question is: why on earth would we write yet another software program for generating a count matrix from single-cell RNA-seq reads when there are already plenty of programs out there? There’s alevin, cell rangerdropseqpipedropseqtoolsindrops… I’ve been going in alphabetical order but have to jump in with starsolo because it’s got the coolest name…now back to optimus, scruff, scpipescumiumis, zumis,  and I’m probably forgetting a few other something-umis. So why another one?

The answer requires briefly venturing back to a time long, long ago when RNA-seq was a fledgling, exciting new technology (~2009). At the time the notion of an “equivalence class” was introduced to the field (see e.g. Jiang and Wong, 2009 or Nicolae et al., 2011). Briefly, there is a natural equivalence relation on the set of reads in an RNA-seq experiment, where two reads are related when they are compatible with (i.e. could have originated from) exactly the same set of transcripts. The equivalence relation partitions the reads into equivalence classes, and, in a slight abuse of notation, the term “equivalence class” in RNA-seq is used to denote the set of transcripts corresponding to an equivalence class of reads. Starting with the pseudoalignment program kallisto that we published in Bray et al. 2016, it became possible to rapidly obtain the (transcript) equivalence classes for reads from an RNA-seq experiment.

When single-cell RNA-seq started to scale it became apparent to those of us working with equivalence classes for bulk RNA-seq that rather than counting genes from single-cell RNA-seq data, it would be better to examine what we called transcript compatibility counts (TCCs), i.e. counts of the equivalence classes (the origin of the term TCC is discussed in a previous blog post of mine). This vision has borne out: we recently published a paper demonstrating the power of TCCs for differential analysis of single-cell data (Ntranos, Yi et al. 2019) and I believe TCCs are ideal for many different single-cell RNA-seq analyses. So back to the question: why another single-cell RNA-seq pre-processing workflow?

Already in 2016 we wanted to be able to produce TCC matrices from single-cell RNA-seq data but there was no program to do it. My postdoc at the time, Vasilis Ntranos, developed a workflow, but in the course of working on a program he started to realize that there were numerous non-trivial aspects to processing single-cell RNA-seq. Even basic questions, such as how to correct barcodes or collapse UMIs required careful thought and analysis. As more and more programs for single-cell RNA-seq pre-processing started to appear, we examined them carefully and noted two things: 1. Most were not able to output TCC matrices and 2. They were, for the most part, based on ad hoc heuristics and unvalidated methods. Many of the programs were not even released with a preprint or paper. So we started working on the problem.

A key insight was that we needed a new format to allow for modular pre-processing. So we developed such a format, which we called the Barcode, UMI, Set (BUS) format, and we published a paper about it earlier this year (Melsted, Ntranos et al., 2019). This allowed us to start investigating different algorithms for the key steps, and to rearrange them and plug them in to an overall workflow as needed. Finally, after careful consideration of each of the key steps, weighing tradeoffs between efficiency and accuracy, and extensive experimentation, we settled on a workflow that is faster than any other method and based on reason rather than intuition. The workflow uses two programs, kallisto and bustools, and we call it the kallisto|bustools workflow. Some highlights:

• kallisto|bustools can produce a TCC matrix. The matrix is compatible with the gene count matrix (it collapses to the latter), and of course gene count matrices can be output as well for use in existing downstream tools.
• The workflow is very very fast. With kallisto|bustools very large datasets can be processed in minutes. The title of this post refers to the workflow as “near-optimal” because it runs in time similar to the unix word count function. Maybe it’s possible to be a bit faster with some optimizations, but probably not by much:
• kallisto|bustools uses very little memory. We worked hard to achieve this feature, as we wanted it to be useful for large-scale analyses that are going to be performed by consortia such as the Human Cell Atlas project. The workflow currently uses ~3.5Gb of RAM for processing 10x v2 chemistry data, and ~11Gb RAM for 10x v3 chemistry data; both numbers are independent of the number of reads being processed. This means users can pre-process data on a laptop:
• The workflow is modular, thanks to its reliance on the flexible BUS format. It was straightforward to develop an RNA velocity workflow (more on this in a companion blog post). It will be easy to adapt the workflow to various technologies, to multiomics experiments, and to any custom analysis required:
• We tried to create comprehensive, yet succinct documentation to help make it easy to use the software (recommendations for improvements are welcome). We have online tutorials, as well as videos for novices:
Installation instructions (and video)
Getting started tutorial (and video).
– Manuals for kallisto and bustools.
– Complete code for reproducing all the results in the preprint
• We were not lazy. In our tests we found variability in performance on different datasets so we tested the program extensively and ran numerous benchmarks on 10x Genomics data to validate Cell Ranger with respect to kallisto|bustools (note that Cell Ranger’s methods have been neither validated nor published). We compiled a benchmark panel consisting of 20 datasets from a wide variety of species. This resulted in 20 supplementary figures, each with 8 panels showing: a) the number of genes detected, b) concordance in counts per gene, c) number of genes detected, d) correlation in gene counts by cell, e) spatial separation between corresponding cells vs. neighboring cells, f,g) t-SNE analysis, h) gene set analysis to detect systematic differences in gene abundance estimation (see example below for the dataset SRR8257100 from the paper Ryu et al., 2019). We also examined in detail results on a species mixing experiment, and confirmed that Cell Ranger is consistent with kallisto on that as well. One thing we did not do in this paper is describe workflows for different technologies but such workflows and accompanying tutorials will be available soon:
• In addition we ran a detailed analysis on the 10x Genomics 10k E18 mouse brain dataset to investigate whether Cell Ranger pre-processing produces different results than kallisto insofar as downstream analyses are concerned. We looked at dimensionality reduction, clustering, identification of marker genes, marker gene prevalence, and pseudotime. The results were all highly concordant. An example (the pseudotime analysis) is shown below:
• We did the math on some of the basic aspects of single-cell RNA-seq. We’re not the first to do this (see, e.g. Petukhov et al., 2018), but one new result we have is an estimation of the UMI diversity on beads. This should be useful for those developing new technologies, or trying to optimize existing protocols:

Note that this post is the first in a series of five that discuss in more detail various aspects of the paper (see links at the top). Finally, a note on reproducibility and usability:

The development of the kallisto|bustools workflow, research into the methods, compilation of the results, and execution of the project required a tremendous team effort, and in working on it I was thinking of the first bioinformatics tool I wrote about and posted to the arXiv (the bioRxiv didn’t exist yet). The paper was:

Nicolas Bray and Lior Pachter, MAVID: Constrained ancestral alignment of multiple sequences, arXiv, 2003.

At the time we posted the code on our own website (now defunct, but accessible via the Wayback machine). We did our best to make the results reproducible but we were limited in our options with the tools available at the time. Furthermore, posting the preprint was highly unusual; there was almost no biology preprinting at the time. Other things have stayed the same. Considerations of software portability, efficiency and documentation were relevant then and remain relevant now.

Still, there has been an incredible development in the tools and techniques available for reproducibility and usability since that time. A lot of the innovation has been made possible by cloud infrastructure, but much of the development has been the result of changes in community standards and requirements (see e.g., Weber et al., 2019). I thought I’d compile a list of the parts and pieces of the project; they are typical for what is needed for a bioinformatics project today and comparing them to the bar in 2003 is mind boggling:

Software: GitHub repositories (kallisto and bustools); releases of binaries for multiple operating systems (Mac, Linux, Windows, Rock64); portable source code with minimal dependencies; multithreading; memory optimization; user interface.

Paper: Preprint (along with extensive Supplement providing backup for every result and claim in the main text); GitHub repository with code to reproduce all the figures/results in the preprint (reproducibility code includes R markdown, python notebooks, snakemake, software versions for every program used, fixed seeds).

Documentation: Manuals for the software; Tutorials for learning to use the code; Explanatory videos (all required materials posted on Github or available on stable websites for download).

The totality of work required to do all of this was substantial. Páll Melsted was the primary developer of kallisto and he wrote and designed bustools, which has been the foundation of the project. The key insight to adopt the BUS format was work in collaboration with Vasilis Ntranos. This was followed by long conversations on the fundamentals of single-cell RNA-seq with Jase Gehring. Sina Booeshaghi carried the project. He was responsible for the crucial UMI collapsing analysis, and put together the paper. Fan Gao, director of the Caltech Bioinformatics Resource Center, set up and implemented the extensive benchmarking, and helped fine-tune the algorithms and converge to the final approach taken. Lambda Lu conducted what I believe to be the most in-depth and detailed analysis to date of the effect of pre-processing on results. Her framework should serve as a template for future development work in this area. Eduardo Beltrame designed the benchmark panels and had a key insight about how to present results that is described in more detail in the companion post on rotating the knee plot. He also helped in the complex task of designing and building the companion websites for the project. Kristján Eldjarn Hjörleifsson helped with the RNA velocity work and helped make custom indices that turned out to be fundamental in understanding the performance of pseudoalignment in the single-cell RNA-seq setting. Sina Booeshaghi spent a lot of time thinking about how to optimize the user experience, making the tutorials and videos, and working overall to make the results of the paper not just reproducible, but the the methods usable.

The long-standing practice of data sharing in genomics can be traced to the Bermuda principles, which were formulated during the human genome project (Contreras, 2010). While the Bermuda principles focused on open sharing of DNA sequence data, they heralded the adoption of other open source standards in the genomics community. For example, unlike many other scientific disciplines, most genomics software is open source and this has been the case for a long time (Stajich and Lapp, 2006). The open principles of genomics have arguably greatly accelerated progress and facilitated discovery.

While open sourcing has become de rigueur in genomics dry labs, wet labs remain beholden to commercial instrument providers that rarely open source hardware or software, and impose draconian restrictions on instrument use and modification. With a view towards joining others who are working to change this state of affairs, we’ve posted a new preprint in which we describe an open source syringe pump and microscope system called poseidon:

A. Sina Booeshaghi, Eduardo da Veiga Beltrame, Dylan Bannon, Jase Gehring and Lior Pachter,

The poseidon system consists of

• A syringe pump that can operate at a wide range of flow rates. The bulk cost per pump is $37.91. A system of three pumps that can be used for droplet based single-cell RNA-seq experiments can be assembled for$174.87
• A microscope system that can be used to evaluate the quality of emulsions produced using the syringe pumps. The cost is $211.69. • Open source software that can be used to operate four pumps simultaneously, either via a Raspberry Pi (that is part of the microscope system) or directly via a laptop/desktop. Together, these components can be used to build a Drop-seq rig for under$400, or they can be used piecemeal for a wide variety of tasks. Along with describing benchmarks of poseidon, the preprint presents design guidelines that we hope can accelerate both development and adoption of open source bioinstruments. These were developed while working on the project; some were borrowed from our experience with bioinformatics software, while others emerged as we worked out kinks during development. As is the case with software, open source is not,  in and of itself, enough to make an application usable.  We had to optimize many steps during the development of poseidon, and in the preprint we illustrate the design principles we converged on with specific examples from poseidon.

The complete hardware/software package consists of the following components:

We benchmarked the system thoroughly and it has similar performance to a commercial Harvard Apparatus syringe pump; see panel (a) below. The software driving the pumps can be used for infusion or withdrawl, and is easily customizable. In fact, the ability to easily program arbitrary schedules and flow rates without depending on vendors who frequently charge money and require firmware upgrades for basic tasks, was a major motivation for undertaking the project. The microscope is basic but usable for setting up emulsions. Shown in panel (b) below is a microfluidic droplet generation chip imaged with the microscope. Panel (c) shows that we have no trouble generating uniform emulsions with it.

Together, the system constitutes a Drop-seq rig (3 pumps + microscope) that can be built for under $400: We did not start the poseidon project from scratch. First of all, we were fortunate to have some experience with 3D printing. Shortly after I started setting up a wet lab, Shannon Hateley, a former student in the lab, encouraged me to buy a 3D printer to reduce costs for basic lab supplies. The original MakerGear M2 we purchased has served us well saving us enormous amounts of money just as Shannon predicted, and in fact we now have added a Prusa printer: The printer Shannon introduced to the lab came in handy when, some time later, after starting to perform Drop-seq in the lab, Jase Gehring became frustrated with the rigidity commercial syringe pumps he was using. With a 3D printer available in-house, he was able to print and assemble a published open source syringe pump design. What started as a hobby project became more serious when two students joined the lab: Sina Booeshaghi, a mechanical engineer, and Eduardo Beltrame, an expert in 3D printing. We were also encouraged by the publication of a complete Drop-seq do-it-yourself design from the Satija lab. Starting with the microscope device from the Stephenson et al. paper, and the syringe pump from the Wijnen et al. paper, we worked our way through numerous hardware design optimizations and software prototypes. The photo below shows the published work we started with at the bottom, the final designs we ended up with at the top, and intermediate versions as we converged on design choices: In the course of design we realized that despite a lot of experience developing open source software in the lab, there were new lessons we were learning regarding open-source hardware development, and hardware-software integration. We ended up formulating six design principles that we explain in detail in the preprint via example of how they pertained to the poseidon design: We are hopeful that these principles we adhered to will serve as useful guidelines for others interested in undertaking open source bioinstrumentation projects. Earlier this month I posted a new paper on the bioRxiv: Jase Gehring, Jeff Park, Sisi Chen, Matt Thomson, and Lior Pachter, Highly Multiplexed Single-Cell RNA-seq for Defining Cell Population and Transciptional Spaces, bioRxiv, 2018. The paper offers some insights into the benefits of multiplex single-cell RNA-Seq, a molecular implementation of information multiplexing. The paper also reflects the benefits of a multiplex lab, and the project came about thanks to Jase Gehring, a multiplex molecular biologist/computational biologist in my lab. mult·i·plex /`məltəˌpleks/ adjective – consisting of many elements in a complex relationship. – involving simultaneous transmission of several messages along a single channel of communication. Conceptually, Jase’s work presents a method for chemically labeling cells from multiple samples with DNA nucleotides so that samples can be pooled prior to single-cell RNA-Seq, yet cells can subsequently be associated with their samples of origin after sequencing. This is achieved by labeling all cells from a sample with DNA that is unique to that sample; in the figure below colors are used to represent the different DNA tags that are used for each sample: This is analogous to the barcoding of transcripts in single-cell RNA-Seq, that allows for transcripts from the same cell of origin to be associated with each other, yet in this framework there is an additional layer of barcoding of cells. The tagging mechanism is a click chemistry one-pot, two-step reaction in which cell samples are exposed to methyltetrazine-activated DNA (MTZ-DNA) oligos as well as the amine-reactive cross-linker NHS-trans-cyclooctene (NHS-TCO). The NHS functionalized oligos are formed in situ by reaction of methyltetrazine with trans-cyclooctene (the inverse-electron demand Diels-Alder (IEDDA) reaction). Nucleophilic amines present on all proteins, but not nucleic acids, attack the in situ-formed NHS-DNA, chemoprecipitating the functionalized oligos directly onto the cells: MTZ-DNAs are made by activating 5′-amine modified oligos with NHS-MTZ for the IEDDA reaction, and they are designed with a PCR primer, a cell tag (a unique “barcode” sequence) and a poly-A tract so that they can be captured by poly-T during single-cell RNA-Seq: Such oligos can be readily ordered from IDT. We are careful to refer to the identifying sequences in these oligos as cell tags rather than barcodes so as not to confuse them with cell barcodes which are used in single-cell RNA-Seq to associate transcripts with cells. The process of sample tagging for single-cell RNA-Seq is illustrated in the figure below. It shows how the tags, appearing as synthetic “transcripts” in cells, are captured during 3′ based microfluidic single-cell RNA-Seq and are subsequently deciphered by sequencing a tag library alongside the cDNA library: This significance of multiplexing is manifold. First, by labeling cells prior to performing single-cell RNA-Seq, multiplexing allows for controlling a trade off between the number of cells assayed per sample, and the total number of samples analyzed. This allows for leveraging the large number of cells that can be assayed with current technologies to enable complex experimental designs based on many samples. In our paper we demonstrate this by performing an experiment consisting of single-cell RNA-Seq of neural stem cells (NSCs) exposed to 96 different combinations of growth factors. The experiment was conducted in collaboration with the Thomson lab that is interested in performing large-scale perturbation experiments to understand cell fate decisions in response to developmental signals. We examined NSCs subjected to different concentrations of Scriptaid/Decitabine, epidermal growth factor/basic fibroblast growth factor, retinoid acid, and bone morphogenic protein 4. In other words, our experiment corresponded to a 4x4x6 table of conditions, and for each condition we performed a single-cell RNA-Seq experiment (in multiplex). This is one of the largest (in terms of samples) single-cell RNA-Seq experiments to date: a 100-fold decrease in the number of cells we collected per sample allowed us to perform an experiment with 100x more samples. Without multiplexing, an experiment that cost us ~$7,000 would cost a few hundred thousand dollars, well outside the scope of what is possible in a typical lab. We certainly would have not been able to perform the experiment without multiplexing. Although the cost tradeoff is impactful, there are many other important implications of multiplexing as well:

• Whereas simplex single-cell RNA-Seq is descriptive, focusing on what is in a single sample, multiplex single-cell RNA-Seq allows for asking how? For example how do cell states change in response to perturbations? How does disease affect cell state and type?
• Simplex single-cell RNA-Seq leads to systematics arguments about clustering: when do cells that cluster together constitute a “cell type”? How many clusters are real? How should clustering be performed? Multiplex single-cell RNA-Seq provides an approach to assigning significance to clusters via their association with samples. In our paper, we specifically utilized sample identification to determine the parameters/thresholds for the clustering algorithm:On the left hand side is a t-SNE plot labeled by different samples, and on the right hand side de novo clusters. The experiment allowed us to confirm the functional significance of a cluster as a cell state resulting from a specific range of perturbation conditions.
• Multiplexing reduces batch effect, and also makes possible the procurement of more replicates in experiments, an important aspect of single-cell RNA-Seq as noted by Hicks et al. 2017.
• Multiplexing has numerous other benefits, e.g. allowing for the detection of doublets and their removal prior to analysis. This useful observation of Stoeckius et al. makes possible higher-throughput single-cell RNA-Seq. We also found an intriguing relationship between tag abundance and cell size. Both of these phenomena are illustrated in one supplementary figure of our paper that I’m particularly fond of:

It shows a multiplexing experiment in which 8 different samples have been pooled together. Two of these samples are human-only samples, and two are mouse-only. The remaining four are samples in which human and mouse cells have been mixed together (with 2,3,4 and 5 tags being used for each sample respectively). The t-SNE plot is made from the tag counts, which is why the samples are neatly separated into 8 clusters. However in Panel b, the cells are colored by their cDNA content (human, mouse, or both). The pure samples are readily identifiable, as are the mixed samples. Cell doublets (purple) can be easily identified and therefore removed from analysis. The relationship between cell size and tag abundance is shown in Panel d. For a given sample with both human and mouse cells (bottom row), human cells give consistently higher sample tag counts. Along with all of this, the figure shows we are able to label a sample with 5 tags, which means that using only 20 oligos (this is how many we worked with for all of our experiments) it is possible to label ${20 \choose 5} = 15,504$ samples.

• Thinking about hundreds (and soon thousands) of single-cell experiments is going to be complicated. The cell-gene matrix that is the fundamental object of study in single-cell RNA-Seq extends to a cell-gene-sample tensor. While more complicated, there is an opportunity for novel analysis paradigms to be developed. A hint of this is evident in our visualization of the samples by projecting the sample-cluster matrix. Specifically, the matrix below shows which clusters are represented within each sample, and the matrix is quantitative in the sense that the magnitude of each entry represents the relative abundance of cells in a sample occupying a given cluster:
A three-dimensional PCA of this matrix reveals interesting structure in the experiment. Here each point is an entire sample, not a cell, and one can see how changes in factors move samples in “experiment space”:

As experiments become even more complicated, and single-cell assays become increasingly multimodal (including not only RNA-Seq but also protein measurements, methylation data, etc.) development of a coherent mathematical framework for single-cell genomics will be central to interpreting the data. As Dueck et al. 2015 point out, such analysis is likely to not only be mathematically interesting, but also functionally important.

We aren’t the only group thinking about sample multiplexing for single-cell RNA-Seq. The “demuxlet” method by Kang et al., 2017 is an in silico approach based on multiplexing from genomic variation. Kang et al. show that if pooled samples are genetically heterogeneous, genotype data can be used to separate samples providing an effective solution for multiplexing single-cell RNA-Seq in large human studies. However demuxlet has limitations, for example it cannot be used for samples from a homogenous genetic background. Two papers at the end of last year develop an epitope labeling strategy for multiplexing: Stoeckius et al. 2017 and Peterson et al. 2017. While epitope labeling provides additional information that can be of interest, our method is more universal in that it can be used to multiplex any kind of samples, even from different organisms (a point we make with the species mixing multiplex experiment I described above). The approaches are also not exclusive, epitope labeling could be coupled to a live cell DNA tagging multiplex experiment allowing for the same epitopes to be assayed together in different samples. Finally, our click chemistry approach is fast, cheap and convenient, immediately providing multiplex capability for thousands, or even hundreds of thousands of samples.

One interesting aspect of Jase’s multiplexing paper is that the project it describes was itself a multiplexing experiment of sorts. The origins of the experiment date to 2005 when I was awarded tenure in the mathematics department at UC Berkeley. As is customary after tenure trauma, I went on sabbatical for a year, and I used that time to ponder career related questions that one is typically too busy for. Questions I remember thinking about: Why exactly did I become a computational biologist? Was a mathematics department the ideal home for me? Should I be more deeply engaged with biologists? Were the computational biology papers I’d been writing meaningful? What is computational biology anyway?

In 2008, partly as a result of my sabbatical rumination but mostly thanks to the encouragement and support of Jasper Rine, I changed the structure of my appointment and joined the UC Berkeley Molecular and Cell Biology (MCB) department (50%). A year later, I responded to a call by then Dean Mark Schlissel and requested wet lab space in what was to become the Li Ka Shing Center at UC Berkeley. This was not a rash decision. After working with Cole Trapnell on RNA-Seq I’d come to the conclusion that a small wet lab would be ideal for our group to better learn the details of the technologies we were working on, and I felt that practicing them ourselves would ultimately be the best way to arrive at meaningful (computational) methods contributions. I’d also visited David Haussler‘s wet lab where I met Jason Underwood who was working on FragSeq at the time. I was impressed with his work and what I saw were important benefits of real contact between wet and dry, experiment and computation.

In 2011 I was delighted to move into my new wet lab. The decision to give me a few benches was a bold and unexpected one, spearheaded by Mark Schlissel, but also supported by a committee he formed to decide on the make up of the building. I am especially grateful to John Ngai, Art Reingold and Randy Scheckman for their help. However I was in a strange position starting a wet lab as a tenured professor. On the one hand the security of tenure provided some reassurance that a failure in the wet lab would not immediately translate to a failure of career. On the other hand, I had no startup funds to buy all the basic infrastructure necessary to run a lab. CIRM, Mark Schlissel, and later other senior faculty in Molecular & Cell Biology at UC Berkeley, stepped in to provide me with the basics: a -80 and -20, access to a shared cold room, a Bioanalyzer (to be shared with others in the building), and a thermocycler. I bought some other basic equipment but the most important piece was the recruitment of my first MCB graduate student: Shannon Hateley. Shannon and I agreed that she would set up the lab and also be lab manager, while I would supervise purchasing and other organization lab matters. I obtained informed consent from Shannon prior to her joining my lab, for what would be a monumental effort requested of her. We also agreed she would be co-advised by another molecular biologist “just in case”.

With Shannon’s work and then my second molecular biology student, Lorian Schaeffer, the lab officially became multiplexed. Jase, who initiated and developed not only the molecular biology but also the computational biology of Gehring et al. 2018 is the latest experimentalist to multiplex in our group. However some of the mathematicians now multiplex as well. This has been a boon to the research of the group and I see Jase’s paper as fruit that has grown from the diversity in the lab. Moving forward, I see increasing use of mathematics ideas in the development of novel molecular biology. For example, current single-cell RNA-Seq multiplexing is a form of information multiplexing that is trivial in comparison to the multiplexing ideas from information theory; the achievements are in the molecular implementations, but in the future I foresee much more of a blur between wet and dry and increasingly sophisticated mathematical ideas being implemented with molecular biology.

Hedy Lamarr, the mother of multiplexing.

The idea that 2016 was the worst year in history was already floated back in July, but despite a lot of negativity, there were definitely good things that happened in 2016. This was certainly true in terms of scientific discoveries and breakthroughs. One of my favorites was a significant improvement to the upper bound of the Happy Ending problem after 81 years!

The Happy Ending problem was the brainchild of mathematician Esther Klein, who proved that any five points in general position in the plane must contain a convex quadrilateral.

The extension of the problem asks, for each n, for the minimum number $f(n)$ so that any $f(n)$ points in general position in the plane contain a convex n-gon. Paul Erdös and George Szekeres established an upper bound for $f(n)$ via Ramsey Theory in 1935, a link that highlighted the importance and application of Ramsey Theory to extremal combinatorics and instantly made the problem a classic. It even has connections to computational biology, via it’s link to the Erdös-Szekeres monotone subsequence theorem (published in the same 1935 paper), which in turn is related to the Needleman-Wunsch algorithm.

The “happy ending” associated to the problem comes from the story of Esther Klein and George Szekeres, who fell in love while working on the problem and ended up marrying in 1937, a happy ending that lasted for 68 years (they died within an hour of each other in 2005).

In their 1935 paper, Erdös-Szekeres proved an upper bound for $f(n)$ using elementary combinatorics, namely

$f(n) \leq {2n -4 \choose n-2}+1 = 4^{n-o(n)}$.

The difficulty of improving the upper bound for $f(n)$ can be appreciated by noting that it took 63 years for there to be any improvement to the Erdös-Szekeres result, and when an improvement did arrive in 1998 it was to remove the “+1” (Fan Chung and Ron Graham, Discrete Geometry 1998) reducing the upper bound to $f(n) \leq {2n-4 \choose n-2}$ for $n \geq 4$.

Considering that the best lower bound for $f(n)$, conjectured to be optimal, is $f(n) \geq 2^{n-2}+1$ (proved by Erdös and Szekeres in 1960), the +1 improvement left a very long way to go. A few other improvements after the Chung-Graham paper was published reduced the upper bound some more, but at most by constant factors.

Then, earlier this year, Andrew Suk announced an astonishing improvement. Building on a theorem of Attila Pór and Pavel Valtr, he proved that

$f(n) = 2^{n+o(n)}$.

The result doesn’t exactly match the conjectured lower bound, so one cannot say the happy ending to the Happy Ending Problem arrived in 2016, but it’s so so so close (i.e. it solves the problem asymptotically) that one can only be left with optimism and hope for 2017.

Happy new year!

One of my favorite systems biology papers is the classic “Stochastic Gene Expression in a Single Cell” by Michael Elowitz, Arnold J. Levine, Eric D. Siggia and Peter S. Swain (in this post I refer to it as the ELSS paper).

What I particularly like about the paper is that it resulted from computational biology flipped. Unlike most genomics projects, where statistics and computation are servants of the data, in ELSS a statistical idea was implemented with biology, and the result was an elegant experiment that enabled a fundamental measurement to be made.

The fact the ELSS implemented a statistical idea with biology makes the statistics a natural starting point for understanding the paper. The statistical idea is what is known as the law of total variance. Given a random (response) variable $C$ with a random covariate  $Z$, the law of total variance states that the variance of $C$ can be decomposed as:

$Var[C] = E_Z[Var[C|Z]] + Var_Z[E[C|Z]]$.

There is a biological interpretation of this law that also serves to explain it: If the random variable $C$ denotes the expression of a gene in a single cell ($C$ being a random variable means that the expression is stochastic), and $Z$ denotes the (random) state of a cell, then the law of total variance describes the “total noise” $Var[C]$ in terms of what can be considered “intrinsic” (also called “unexplained”) and “extrinsic” (also called “explained”) noise or variance.

To understand intrinsic noise, first one understands the expression $Var[C|Z]$ to be the conditional variance, which is also a random variable; its possible values are the variance of the gene expression in different cell states. If $Var[C]$ does not depend on $Z$ then the expression of the gene is said to be homoscedastic, i.e., it does not depend on cell state (if it does then it is said to be heteroscedastic). Because $Var[C|Z]$ is a random variable, the expression $E_Z[Var[C|Z]$ makes sense, it is simply the average variance (of the gene expression in single cells) across cell states (weighted by their probability of occurrence), thus the term “intrinsic noise” to describe it.

The expression $E[C|Z]$ is a random variable whose possible values are the average  of the gene expression in cells. Thus, $Var_Z[E[C|Z]]$ is the variance of the averages; intuitively it can be understood to describe the noise arising from different cell state, thus the term “extrinsic noise” to describe it (see here for a useful interactive visualization for exploring the law of total variance).

The idea of ELSS was to design an experiment to measure the extent of intrinsic and extrinsic noise in gene expression by inserting two identically regulated reporter genes (cyan fluorescent protein and yellow fluorescent protein) into E. coli and measuring their expression in different cells. What this provides are measurements from the following model:

Random cell states are represented by random variables $Z_1,\ldots,Z_n$ which are independent and identically distributed, one for each of n different cells, while random variables $C_1,\ldots,C_n$  and $Y_1,\ldots,Y_n$ correspond to the gene expression of the cyan , respectively yellow, reporters in those cells. The ELSS experiment produces a single sample from each variable $C_i$ and $Y_i$, i.e. a pair of measurements for each cell. A hierarchical model for the experiment, in which the marginal (unconditional) distributions $C_i$ and $Y_i$ are identical, allows for estimating the intrinsic and extrinsic noise from the reporter expression measurements.

The model above, on which ELSS is based, was not described in the original paper (more on that later). Instead,  in ELSS the following estimates for intrinsic, extrinsic and total noise were simply written down:

$\eta^2_{int} = \frac{\frac{1}{n} \left( \sum_{i=1}^n \frac{1}{2} (c_i-y_i)^2\right) }{\overline{c} \cdot \overline{y}},$ (intrinsic noise)

$\eta^2_{ext} = \frac{\frac{1}{n}\sum_{i=1}^n c_i \cdot y_i - \overline{c} \cdot \overline{y}}{\overline{c} \cdot \overline{y}},$ (extrinsic noise)

$\eta^2_{tot} = \frac{\frac{1}{n}\sum_{i=1}^n \frac{1}{2} (c_i^2+y_i^2)-\overline{c}\cdot\overline{y}}{\overline{c} \cdot \overline{y}}.$ (total noise)

Here  $c_1,\ldots,c_n$ and $y_1,\ldots,y_n$ are the measurements of cyan respectively yellow reporter expression in each cell, $\overline{c} = \frac{1}{n}\sum_{i=1}^n c_i$ and $\overline{y} = \frac{1}{n}\sum_{i=1}^n y_i$.

Last year, Audrey Fu, at the time a visiting scholar in Jonathan Pritchard’s lab and now assistant professor in statistical science at the University of Idaho,  studied the ELSS paper as part of a journal club. She noticed some inconsistencies with the proposed estimates in the paper, e.g. it seemed to her that some were biased, whereas others were not, and she proceeded to investigate in more detail the statistical basis for the estimates. There had been a few papers trying to provide statistical background, motivation and interpretation for the formulas in ELSS (e.g. A. Hilfinger and J. Paulsson, Separating intrinsic from extrinsic fluctuations in dynamic biological systems, 2011 ), but there had not been an analysis of bias, or for that matter other statistical properties of the estimates. Audrey started to embark on a post-publication peer review of the paper, and having seen such reviews on my blog contacted me to ask whether I would be interested to work with her. The project has been a fun hobby of ours for the past couple of months, eventually leading to a manuscript that we just posted on the arXiv:

Audrey Fu and Lior Pachter, Estimating intrinsic and extrinsic noise from single-cell gene expression measurements, arXiv 2016.

Our work provides what I think of as a “statistical companion” to the ELSS paper. First, we describe a formal hierarchical model which provides a useful starting point for thinking about estimators for intrinsic and extrinsic noise. With the model we re-examine the ELSS formulas, derive alternative estimators that either minimize bias or mean squared error, and revisit the intuition that underlies the extraction of intrinsic and extrinsic noise from data. Details are in the paper, but I briefly review some of the highlights here:

Figure 3a of the ELSS paper shows a scatterplot of data from two experiments, and provides a geometric interpretation of intrinsic and extrinsic noise that can guide intuition about them. We have redrawn their figure (albeit with a handful of points rather than with real data) in order to clarify the connections to the statistics:

The Elowitz et al. caption correctly stated that “Each point represents the mean fluorescence intensities from one cell. Spread of points perpendicular to the diagonal line on which CFP and YFP intensities are equal corresponds to intrinsic noise, whereas spread parallel to this line is increased by extrinsic noise”. While both statements are true, the one about intrinsic noise is precise whereas the one about extrinsic noise can be refined. In fact, the ELSS extrinsic noise estimate is the sample covariance (albeit biased due to a prefix of n in the denominator rather than n-1), an observation made by Hilfinger and Paulsson. The sample covariance has a  (well-known) geometric interpretation: Specifically, we explain that it is the average (signed) area of triangles formed by pairs of data points (one the blue one in the figure): green triangles in Q1 and Q3 (some not shown) represent a positive contribution to the covariance and magenta triangles in Q2 and Q4 represent a negative contribution. Since most data points lie in the 1st (Q1) and 3rd (Q3) quadrants relative to the blue point, most of the contribution involving the blue point is positive. Similarly, since most pairs of data points can be connected by a positively signed line, their positive contribution will result in a positive covariance. We also explain why naïve intuition of extrinsic noise as the variance of points along the line $c=y$ is problematic.

The estimators we derive are summarized in the table below (Table 1 from our paper):

There is a bit of algebra that is required to derive formulas in the table (see the appendices of our paper). The take home messages are that:

1. There is a subtle assumption underlying the ELSS intrinsic noise estimator that makes sense for the experiments in the ELSS paper, but not for every type of experiment in which the ELSS formulas are currently used. This has to do with the mean expression level of the two reporters, and we provide a rationale and criteria when to apply quantile normalization to normalize expression to the data.
2. The ELSS intrinsic noise estimator is unbiased, whereas the ELSS extrinsic noise estimator is (slightly) biased. This asymmetry can be easily rectified with adjustments we derive.
3. The standard unbiased estimator for variance (obtained via the Bessel correction) is frequently, and correctly, criticized for trading off mean squared error for bias. In practice, it can be more important to minimize mean squared error (MSE). For this reason we derive MSE minimizing estimators. While the MSE minimizing estimates converge quickly to the unbiased estimates (as a function of the number of cells), we believe there may be applications of the law of total variance to problems in systems biology where sample sizes are smaller, in which case our formulas may become useful.

The ELSS paper has been cited more than 3,000 times and with the recent emergence of large scale single-cell RNA-Seq the ideas in the paper are more relevant than ever. Hopefully our statistical companion to the paper will be useful to those seeking to obtain a better understanding of the methods, or those who plan to extend and apply them.

The hierarchical classification of nature initiated by Carl Linnaeus today consists of eight major “ranks”, namely species, genus, family, order, class, phylum, kingdom and domain:

In the microbial world it makes sense to refine the standard taxonomy by subdividing species into strains. An important reason to do so is that bacterial taxonomy must reflect not only phylogeny but also pathogenicity, and small differences in genomes can translate to large pathogenic differences. This has implications for metagenomic analyses of microbial communities: for many biomedical applications it is desirable to characterize individuals strains.

Metagenomics has its roots in culture-independent retrieval and sequencing of 16S rRNA genes, and while variations in 16S can sometimes distinguish between strains, a single gene is not always sufficient. This limitation of 16S can be overcome with whole genome shotgun sequencing of microbial communities, an approach to metagenomics that became popular in the early 2000s and  that opened the door to higher resolution characterization of communities. In 2005 Kevin Chen and I wrote a review on the bioinformatics challenges that would have to be overcome to walk through the door. One of the things we did was to emphasize “problems and their connections to other areas of bioinformatics, such as… gene expression analysis”, and throughout the past decade I’ve always hoped for deeper connections to be established between metagenomics and gene expression bioinformatics. I’ve noticed interesting connections pop up from time to time (e.g. Paulson et al. 2013)  and have occasionally entertained the thought with my students and collaborators, especially as work in my group became more focused on RNA-Seq since the development of Cufflinks in 2008.

However connection modern transcriptome analysis methodology, specifically bioinformatics of RNA-Seq to metagenomics has been difficult to do until recently. One major reason is that until just a few years ago, there was no reference genome database for metagenomics analogous to the reference annotation databases available for use in transcriptomics. Another way to put this is that metagenomics has, until recently, been “de novo” bioinformatics. By this I mean that the analysis of communities from whole genome shotgun data had to largely proceed via de novo analyses of the data (e.g. de novo assembly of genomes), “binning” of reads according to sequence characteristics or hits to gene databases was required because it was impossible to compare sequences to references genomes. While de novo methods have also been developed for RNA-Seq, the scale of transcriptome analysis is much smaller than that of most metagenomic analyses, and as has been well documented, de novo transcriptomics is already very difficult (e.g. Amin et al. 2014).

The de novo state of metagenomics has changed in recent years, as (relatively) low-cost sequencing has been a boon for microbial genomics. The graph below, extracted from NCBI and published in a recent review, shows that in just the past few years thousands of bacterial genomes have been sequences, enabling, for the first time, reference based metagenomics:

This observation is reflected in the recent development of many methods for a variety of metagenomic applications that take advantage of reference genome databases.  Specifically, the problem of read assignment, which is fundamental for abundance estimation, has benefited from the possibility of metagenomic read alignment to reference databases.

The figure below, reproduced from the preprint “An evaluation of the accuracy and speed of metagenome analysis tools” by Stinus Lindgreen, Karen L. Adair and Paul Gardner, bioRxiv May 15, 2015 shows a benchmark of the accuracy and runtime of 14 programs developed for metagenomic read assignment for whole genome shotgun data:

The problem these methods are solving is really similar to the problem of read assignment in RNA-Seq. In RNA-Seq, instead of originating from strains, reads originate from transcripts. Just as strains are present in different abundances in a community, so are RNA transcripts in a cell (or in bulk). The analogy of taxonomy in metagenomics, i.e. the grouping of strains into species, genus etc. is also present in RNA-Seq, where transcripts are grouped into genes. The fragment (or read) assignment problem in RNA-Seq is closely related to the quantification problem in RNA-Seq and is a problem that has been thoroughly researched and for which many algorithms have been developed. I discussed the importance of the fragment assignment problem for RNA-Seq in my 2013 Genome Informatics Keynote.

In response to the development of reference-based bioinformatics possibilities for metagenomics, about three years ago my student Lorian Schaeffer started looking at the suitability of RNA-Seq tools for metagenomic read assignment. Although the metagenomic and RNA-Seq assignment problems are conceptually similar and methodologically related, there are various technical issues involved in applying RNA-Seq tools in the metagenomic setting (e.g. the need to carefully account for taxonomy in the metagenomics setting). After developing the computational infrastructure to benchmark RNA-Seq programs in the metagenomic setting, she proceeded to evaluate the accuracy of eXpress, a streaming algorithm for RNA-Seq quantification. Although the quantification of eXpress was specifically designed to be suitable for large numbers of reads, the program requires read alignments to a reference transcriptome (or in Lorian’s experiments a genome) database. In the metagenomic setting realistic databases are huge, and she found that it took days just to map the reads. Nevertheless, her initial benchmarks revealed that eXpress was significantly more accurate than the available metagenomic read assignment tools of the time.

When Kraken (Wood and Salzberg 2014), and later CLARK (Ounit et al. 2015) were published in 2014 and 2015 respectively, we took note because by circumventing the alignment step they dramatically altered the tractability of metagenomic read assignment. In parallel, in my group, Nicolas Bray and later Páll Melsted and Harold Pimentel were developing what is now kallisto (Bray et al. 2015). Like Kraken, kallisto avoided the need for aligning reads, but with the introduction of the concept of pseudoalignment, allowed for accurate read assignments based on joint analysis of exact k-mer matches. What we showed earlier this year is that unlike naïve k-mer based approaches to quantification, kallisto is as accurate as eXpress and other read alignment based quantification tools, and this observation led Lorian to immediately proceed to benchmark it on metagenomic data. The result of her work was just posted as a preprint:

Lorian Schaeffer, Harold Pimentel, Nicolas Bray, Páll Melsted and Lior Pachter, Pseudoalignment for metagenomic read assignment, arXiv 1510.07371, 2015.

With this paper we demonstrate a “technology transfer” from RNA-Seq bioinformatics to metagenomics, one that achieves dramatic improvements in read assignment accuracy in the metagenomics setting. The main result of her work is Table 1 in our preprint:

Using a published simulated Illumina dataset from Mende et al. 2012 (based on 100 genomes and containing 53.33 million reads), and augmenting it with another 2,308 genomes for the purpose of testing, she shows that kallisto significantly outperforms the best quantification methods (as benchmarked by Lindgreen et al., see figure above). “Significant” here refers to what I think is fair to characterize as an extraordinary improvement: at the genus level, a level that programs such as CLARK have been optimized for, kallisto’s RRMSE (relative root mean squared error)  is 0.13 compared to 17.05 for Kraken and 18.58 for CLARK. The improvement is based on two ideas: first, the results show that the model based approach for read assignment, the concept that underlies GASiC and eXpress, outperforms direct taxonomic read assignment as implemented by MEGAN and Kraken and CLARK (in the latter approach reads are aligned to the lowest rank to which they align unambigously). Second, pseudoalignment is not just faster than traditional alignment but also accurate.

The upshot: the accuracy and efficiency of kallisto make strain level analysis of metagenomes possible. In fact kallisto is more accurate at the strain level than other programs are at the genus level. Just as we have been advocating for transcript level analysis from RNA-Seq data, we believe that strain level analysis should become commonplace in metagenomics.

In digging deeply into the bioinformatics of metagenomics bioinformatics we noticed a few other areas that could benefit from RNA-Seq technology transfer. For example, the standard of RNA-Seq methods benchmarking appears to be higher than in metagenomics. Both the Kraken and CLARK papers benchmarked their programs on simulations with 10 genomes (the number ten is not a typo). CLARK did test on one dataset with 20 genomes, although using only 10,000 reads. To be fair to the authors of those papers, their standards were much higher than others in the field. The paper

Yu-Wei Wu and Yuzhen Ye, A novel abundance-based algorithm for binning metagenomic sequences using l-tuples, Journal of Computational Biology 2011.

benchmarked their method on simulations of reads from 2 (two!!) organisms. Biologists frequently complain that simulations of bioinformaticians are completely non-informative and unfortunately these cases provide fodder for such prejudice. Having said that, the RNA-Seq community also has much to learn from the metagenomics community. The previously mentioned paper by Paulson et al. 2013 addresses missing data in a way that should translate directly to missing data in single-cell RNA-Seq (the paper also makes performance comparisons with their comparative metagenomics approach to the RNA-Seq programs DESeq and edgeR) . One paper (McDavid et al. 2012) does take a look at modeling single-cell data with zero inflated distributions but I think this is a good example where metagenomics is ahead of RNA-Seq. Our immediate plans are to develop the kallisto application to metagenomics to include the ability to perform metagenome comparisons using sleuth. Conversely, inspired by the taxonomy hierarchy fundamental to metagenomics we’re going to explore RNA-Seq quantification with groups of transcripts that go beyond just genes.

Horizontal transfer is good.

 Qun Anrikar on Sexual harassment case number… Dmitry Kondrashov on Time to end letter grades Joel Miller on The lethal nonsense of Michael… U. Ploy on The amoral nonsense of Orchid… JC JC on Williams math professor invest… Anonymous on The amoral nonsense of Orchid… John D. Brown Junior on The two cultures of mathematic… ziphon on The amoral nonsense of Orchid… Lior Pachter on The amoral nonsense of Orchid… Craken on The amoral nonsense of Orchid…

### Blog Stats

• 2,658,303 views